epMotion^® 5075t NGS solution

View through epMotion workdeck with TM 300 tool with X50 and Illumina sequencer in background with lab person

NGS Made Easy - The epMotion 5075 NGS solution combines automated liquid handling and unique software features with the accessories and high-quality consumables you need for reliable Next Generation Sequencing library preparation and maximum NGS kit flexibility. Next Generation Sequencing sample preparation is a labor-intensive process, which requires experience, precision and accuracy to generate high-quality NGS libraries. Automating Next Generation Sequencing library preparation eliminates the risk of pipetting errors, provides reproducible results and increases your productivity. A vast list of automated Next Generation Sequencing sample prep methods has been qualified by kit manufacturers and customers to deliver sequencing results that are comparable or better to those from manual preparation. In addition, an intuitive software interface allows for ease-of-use and rapid method design, while providing the needed flexibility to optimize even the most complex Next Generation Sequencing workflows. More information

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NGS methods - available for epMotion automated library prep

Automated pipetting systems: Qualify for a demo

NGS methods - available for epMotion automated library prep

Automated pipetting systems: Qualify for a demo

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epMotion^® 5075t NGS solution, package with completely contained housing, MultiCon PC, Enhanced feature set 1, C2 thermal module, dispensing tools, plus NGS specific accessories, plus NGS specific consumables to start automated library preparation, 100 – 240 V ±10 %/50 – 60 Hz ±5 %, 0.2 µL – 1 mL Catalog No. 5075000962

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epMotion^® 5075tC NGS solution, package with CleanCap, MultiCon PC, Enhanced feature set 1, C2 thermal module, dispensing tools, plus NGS specific accessories, waste bag holder, plus NGS specific consumables to start automated library preparation, 100 – 240 V ±10 %/50 – 60 Hz ±5 %, 0.2 µL – 1 mL Catalog No. 5075000963

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Product Information

The epMotion 5075 NGS solution includes everything to start automated library preparation. The basic system is an epMotion 5075t with an integrated Eppendorf ThermoMixer^® to mix, heat or cool samples and reagents, and a thermal module on deck position C2 for additional temperature control. A gripper for labware transport and 2x single- and 2x 8-channel pipetting tools to cover a volume range from 1 to 300 µL. Next Generation Sequencing specific accessories (Thermoblock 96 OC, 2 x Thermoadapter 96-well, Rack ILMN tubes, ReservoirRack, Eppendorf Magnet Adapter FLX, waste bag holder) are included, plus NGS specific consumables (Eppendorf twin.tec^® LoBind PCR Plate 96 semi-skirted, epT.I.P.S.^® Motion pipette tips with filter PCR clean 50 µL & 300 µL each 960 tips, LiquidWasteTub 400 mL, 30 mL reservoirs, waste bags). The epMotion 5075 NGS solution is available from stock with and without CleanCap option in all different voltage versions.

ThermoMixer^®

Fully integrated ThermoMixer with 2DMix-Control improves yield and application results, e.g. resuspending bacteria pellets or magnetic beads. Peltier element for heating and cooling of sample and/or reagents. The following epMotion versions are equipped with ThermoMixer capability, M5073, 5075t, 5075m, 5075vt. A ThermoMixer upgrade product is available for epMotion 5075l, 5075v, and successor versions 5075LH and 5075VAC.

Sample preparation for Next Generation Sequencing (NGS)

involves extensive manual processing, is highly time-consuming and needs intense concentration. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of pipetting errors.

Gripper Tool

The gripper tool allows transport of labware on the worktable of epMotion 5073 or 5075 versions. It is loaded by automatic tool exchange and can be programmed easily in the software. The stacking of up to 5 plates is possible.

Features

Same as 5075t, plus
Industry-proven, large repertoire of manufacturer and customer qualified Next Generation Sequencing methods guarantees sequencing results comparable or better to those from manual preparation
Intelligent Tool Selection automatically decides which tools (single or 8-channel) to use for fastest run time – ideal when sample numbers change frequently or are not a multiple-of-8
Optimized bead clean-up on magnetic plate adapter to prevent carryover contamination
Email notification option keeps you informed about the status of your library prep run
Increased stacking capabilities for plates and tip boxes for more efficient use of deck space
Integrated, high-performance ThermoMixer and thermal module allow for efficient mixing and reliable temperature control
CleanCap option provides UV decontamination and HEPA air filter to protection samples from contaminations
Automating Next Generation Sequencing library preparation using epMotion provides reproducible results due to its outstanding pipetting accuracy (0.31 % at 1 µL) and precision (1.97 % at 1 µL) and by eliminating the risk of pipetting errors

Applications

Like 5075l, plus
Bead applications with mixing and tempering
Sequencing and PCR clean-up
Nucleic acid purification
Cell disruption
Immunoassays
Next Generation Sequencing library preparation

	epMotion^® 5075t NGS solution	epMotion^® 5075tC NGS solution
Gripper carrying capacity	1,200 g	1,200 g
Mixing frequency	Off, 300 – 2,000 rpm, optimized for labware and application	Off, 300 – 2,000 rpm, optimized for labware and application
Temperature homogeneity	≤ 10 % on the block	≤ 10 % on the block
Application	NGS	NGS
Heating rate	5 °C/min, on the block	5 °C/min, on the block
Cooling rate	3 °C/min, on the block, above RT	3 °C/min, on the block, above RT
Volume range	0.2 µL – 1 mL	0.2 µL – 1 mL
Power supply	100 – 240 V ±10 %, 50 – 60 Hz ±5 %	100 – 240 V ±10 %, 50 – 60 Hz ±5 %
Max. power consumption	700 W	700 W
Dimensions (W × D × H)	107 × 61 × 67 cm / 43 × 24 × 27 in	107 × 61 × 67 cm / 43 × 24 × 27 in
Temperature range	-15 °C below RT to 95 °C measured in labware at 75 % filling height	-15 °C below RT to 95 °C measured in labware at 75 % filling height
Conductor – X, Y, Z Positioning
Random measurement error	±0.1 mm	±0.1 mm
Systematic measurement error	±0.3 mm	±0.3 mm
Detector
Optical confocal infrared detector	Contact-free recognition of fill level, inserted tools, labware, tip types and quantities	Contact-free recognition of fill level, inserted tools, labware, tip types and quantities
Optical sensor	Contact-free recognition of fill level, inserted tools, labware, tip types and quantities	Contact-free recognition of fill level, inserted tools, labware, tip types and quantities
Dispensing tool
Random measurement error (1 µL)^1,2	≤3 %	≤3 %
Random measurement error (1,000 µL)^1,2	≤0.15 %	≤0.15 %
Random measurement error (50 µL)^1,2	≤0.4 %	≤0.4 %
Systematic measurement error (1 µL)^1,2	±5 %	±5 %
Systematic measurement error (1,000 µL)^1,2	±0.7 %	±0.7 %
Systematic measurement error (50 µL)^1,2	±1.2 %	±1.2 %

¹in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °C
²See Eppendorf Application Note 168 for typical pipetting performance

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