Mastercycler® X50

Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50

The new Mastercycler X50 is the elegant synthesis of speed, flexibility and PCR optimization for research PCR applications and standardization for routine PCR applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. More information

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Product Information

The Mastercycler X50 family boasts ramp rates of up to 10 °C/s and is the ideal cycler family for routine applications as well as advanced research in molecular biology.

The Mastercycler X50 family consist of units with a user-friendly VisioNize touchscreen interface or eco units which are externally controlled. Each cycler with a touchscreen can be networked with nine additional eco units. If that is not enough throughput, you can network up fifty Mastercycler eco units to a computer or laptop running CycleManager X50 as a central controller. Whichever you choose, you can independently control and monitor every unit. You can also manage who has access to the system and designate a level of access with our intuitive user interface. Low noise levels, low power consumption, and the versatile flexlid® concept complete the product to be a powerful and convenient PCR cycler.

PCR Optimization of β-actin Gene with 2D-Gradient technique.


2D-Gradient allows you to optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) during the same run. Higher denaturation temperatures can have the advantage of increased specificity while lower denaturation temperatures reduce stress on biomolecules and can lead to increased yield. Assays that struggle to work reliably at a 95° denaturation temperature could benefit significantly from the optimization of the denaturation temperature.

Mastercycler® X50

Results needed asap?


PCR should be faster. That gives you quicker results, throughout the day, and a faster transition to optimal temperatures. The Mastercycler X50 heats with 10 °C/s and cools with 5 °C/s. Make the speed of the Mastercycler X50 your research advantage.

Mastercycler® X50

Checking ramp rates by the stopwatch?


There is a large variety of cyclers available with different ramp rates published.
If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not. For more details, see Application Note 274.

Mastercycler® X50

Annoyed by uncomfortable handling?


Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf Mastercyler:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Mastercycler® X50

Struggling with paper-based documentation?


The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like PCR–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Mastercycler® X50

Features

  • Heating rate: 5 °C/s
  • Wide selection of blocks from a fast silver block to 384 well block
  • VisioNize touchscreen interface
  • Innovative 2D-Gradient for advanced PCR optimization
  • X50 with Touchscreen can control up to 9 other units (connected cyclers do not have a touch screen interface)
  • flexlid® concept: automatic height adjustment of the lid allows you to use all types of consumables
  • Small footprint
  • 2 Year warranty
  • Warranty: 2 Year warranty

Applications

  • Fast PCR
  • PCR optimization
  • Standard PCR
  • Incubation
  • Cycle sequencing
 MC X50sMC X50aMC X50pMC X50hX50iX50lX50rX50t
For use with (application)Fast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencingFast PCR, PCR optimization, Standard PCR, Incubation, Cycle sequencing
Touchscreen Interface
Unit can control 9 other units in network
Unit can only be controlled
ThermoblockSilverAluminumAluminumAluminumSilverAluminumAluminumAluminum
Sample capacity96-well plate or 0.1/0.2 mL tubes96-well plate or 0.1/0.2 mL tubes1 PCR plate (96 wells)1 PCR plate 38496-well plate or 0.1/0.2 mL tubes96-well plate or 0.1/0.2 mL tubes1 PCR plate (96 wells)1 PCR plate 384
96-well plate
384-well plate
0.1/0.2 mL tubes
Heating technologySix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elements
2D-Gradient blockover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rows
Temperature control modeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, Safe
Temperature control range of the block4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C
Gradient range1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C
Gradient temperature range30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C
Block homogeneity 20–72 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C
Block homogeneity 95 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C
Control accuracy±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C
Heating rate10 °C/s5 °C/s5 °C/s5 °C/s10 °C/s5 °C/s5 °C/s5 °C/s
Cooling rate5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s
Lid descentflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSP
High pressure lid
Lid temperature range37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C
InterfacesEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USB
Power supply110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz
Max. power consumption850 W850 W850 W850 W850 W850 W850 W850 W
Total weight11.5 kg11.5 kg11.5 kg11.5 kg10.7 kg10.7 kg10.7 kg10.7 kg
Dimensions (W × D × H)27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm

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